Description
The GenElute™-E Single Spin Blood DNA 96 Kit has been developed to purify genomic DNA from a wide variety of blood samples, including fresh, frozen or whole blood or dried blood spots (FTA card punches). EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used. The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse™ Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8